Tabriz University of Medical Sciences Journal of Dental Research, Dental Clinics, Dental Prospects 2008-210X 8 2 2014 06 11 Sealing Efficacy of Single-cone Obturation Technique with MTA and CEM Cement: An in Vitro Bacterial Leakage Study 77 83 10.5681/joddd.2014.014 EN Mohammad Samiei Mohammad Aghazade Farrokh Farhadi Naeimeh Shahveghar Ali Torab Seyyed Mahdi Vahid Pakdel Journal Article 10.5681/joddd.2014.014 2014 07 17 Background and aims. Various materials and methods have been introduced for obturating cleaned and shaped root canal systems. This in vitro study aimed to evaluate the sealing ability of single-cone obturation technique with mineral tri-oxide aggregate and calcium-enriched mixture based on bacterial leakage approach. Materials and methods. Sixty-four single-canal teeth were prepared and randomly divided into 5 groups, consisting of three experimental groups (n = 16) and two control groups (n = 8).In group 1, root canal obturation was performed using gutta-percha with 0.02 taper and AH26 sealer by lateral compaction technique. In groups 2 and 3, single Protaper gutta-percha cone was used for obturation with MTA and CEM cement, respectively. A bacterial leakage apparatus was utilized for leakage assessment for 60 days. Leakage comparison between the experimental groups was performed by one-way ANOVA using SPSS 16 statistical software. Results. The mean bacterial leakage intervals in groups 1, 2 and 3 were 33.68 ± 9.39, 40.68 ± 11.03 and 39.56 ± 9.03 days, respectively. One-way ANOVA indicated no significant differences in bacterial leakage between the three experimen-tal groups (P = 0.109). Conclusion. Single-cone obturation with well-fitted gutta-percha and MTA and CEM cement is an appropriate alternative for traditional lateral compaction technique.