Tabriz University of Medical Sciences Journal of Dental Research, Dental Clinics, Dental Prospects 2008-210X 19 1 2025 03 31 Effect of ethylenediamine tetraacetic acid (EDTA) and maleic acid as root-end conditioners on the sealing ability of ProRoot mineral trioxide aggregate (MTA) and Biodentine as root-end filling materials: An in vitro study 29 32 10.34172/joddd.025.41232 EN Sumaiya Zafar https://orcid.org/0000-0002-5122-7122 Yawar Ali Abidi https://orcid.org/0000-0002-4122-3572 Samira Adnan https://orcid.org/0000-0002-7866-4339 Maham Muneeb Lone https://orcid.org/0000-0002-9124-5286 Farah Afroz Khan https://orcid.org/0000-0001-8162-7021 Isma Sajjad Journal Article 10.34172/joddd.025.41232 2024 04 23 2025 01 31 Background. Apicoectomy is often required to treat a non-healing endodontic lesion. Materials used during this procedure, such as root-end conditioners and retrograde filling materials, can significantly affect the success. Mineral trioxide aggregate (MTA) has been used as a root-end filling material for a long time; however, success has also been reported with some novel materials such as Biodentine. The present study compared the sealing ability of MTA and Biodentine in root-end cavities following apicoectomy after using EDTA and maleic acid as root-end conditioners. Methods. The apical one-third of 60 extracted teeth were resected, followed by root-end preparation of 3 mm. The samples were divided into 4 groups of 15 each. The roots in groups 1 and 2 were conditioned with EDTA and maleic acid in groups 3 and 4. Retrograde cavities in groups 1 and 3 were filled with MTA and in groups 2 and 4 with Biodentine. Microleakage was checked using the dye penetration method. One-way ANOVA and post hoc Tukey tests were used for statistical analyses. Results. Group 1 showed the highest (2.94±0.112), while group 4 had the least dye penetration (2.55±0.213). Conclusion. It can be concluded that Biodentine showed better sealing ability after using maleic acid than MTA and can be used as an alternative to MTA in root-end surgeries.