Tabriz University of Medical Sciences Journal of Dental Research, Dental Clinics, Dental Prospects 2008-210X 20 3 2026 09 30 Comparative Evaluation of Fluoride Release and Rechargeability in Novel Resin-Based and Various Pit and Fissure Sealants: An in Vitro Study 194 199 10.34172/joddd.42792 EN Pannaros Torsakul https://orcid.org/0000-0001-5249-2092 Chitralada Suparangkarat Chanapa Angsurattanasopon Kunlanan Khanchai Kittitath Joongjai Praphasri Rirattanapong https://orcid.org/0000-0001-7286-0948 Journal Article 10.34172/joddd.42792 2025 06 09 2026 09 11 Introduction: Dental caries is a major global health concern, and the occlusal surfaces of molars are particularly vulnerable because of their deep pits and fissures. Sealants effectively prevent caries on these vulnerable surfaces. Researchers have extensively studied fluoride release from various sealant types, but the fluoride release and rechargeability of novel bioactive resin-based sealants remain unexamined. This study investigated the fluoride release and rechargeability of five sealant materials, with a particular focus on novel bioactive resin-based sealants. Methods: Five dental sealants were evaluated: a non-fluoride-releasing resin-based sealant (Concise™), a fluoride-releasing resin-based sealant (Clinpro™), a bioactive resin-based sealant (BioCoat®), a giomer sealant (BeautiSealant), and a glass ionomer sealant (Fuji VII®). Disk-shaped specimens (11×1 mm) were prepared (n=7 per group) and submerged in 3 mL of deionized water at 37°C. Fluoride release was measured on days 1–7, 14, and 21, using a fluoride ion-selective electrode. After 21 days, the specimens were treated with a 1.23% acidulated phosphate fluoride (APF) gel for 4 minutes, and the fluoride release was evaluated daily for 5 days under the same conditions. Results: Initial fluoride release was highest for Fuji VII®, followed by BeautiSealant, Clinpro™, BioCoat®, and Concise™. After fluoride recharge, Fuji VII® showed the highest release, followed by BeautiSealant, BioCoat®, Clinpro™, and Concise™ (P<0.001). Conclusion: The fluoride-releasing and recharging abilities of bioactive resin-based sealants highlighted their potential as a viable dental alternative for pit and fissure protection.