J Dent Res Dent Clin Dent Prospects. 2014;8(3): 130-133.
doi: 10.5681/joddd.2014.024
PMID: 25346830
PMCID: PMC4206753
  Abstract View: 286
  PDF Download: 471

Original Research

Cytotoxicity Evaluation of Iranian Propolis and Calcium Hydroxide on Dental Pulp Fibroblasts

Maryam Zare Jahromi 1, Parisa Ranjbarian 2, Samaneh Shiravi 3 *

1 Assistant Professor, Department of Endodontics, Faculty of Dentistry, Islamic Azad University Khorasgan (Isfahan) Branch, Isfahan, Iran
2 Post-graduate Student, Department of Endodontics, Faculty of Dentistry, Islamic Azad University, Khorasgan (Isfahan) Branch, Isfahan, Iran
3 Dentist, Private Practice, Isfahan, Iran


Background and aims. Since intracanal medicaments can affect the cell viability in periapical tissues, the aim of this study was to evaluate the effect of calcium hydroxide and propolis on pulp fibroblasts. Materials and methods. Two healthy third molars were used as a source to obtain fibroblasts.  The fibroblasts were cultured and subjected to 1 mg/mL of propolis and calcium hydroxide. This experiment was performed in six replicates and cell viability was evaluated with MTT assay. Statistical analysis was performed by t-test. Results. Comparison of cell viability with the use of 1 mg/mL of calcium hydroxide and propolis showed that cells subjected to propolis were more viable when compared to calcium hydroxide (P < 0.05). Conclusion. In this study, calcium hydroxide reduced fibroblast viability, significantly more than Iranian propolis. Other properties should be evaluated before Iranian propolis could be indicated for use as intracanal medicament.
Keywords: Cytotoxicity, calcium hydroxide, fibroblast, MTT, propolis
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Submitted: 04 Oct 2014
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