Mahmoud Bahari
1,2, Soodabeh Kimyai
3, Firoz Pouralibaba
3, Farrokh Farhadi
4, Marouf Norouzi
5, Siavash Savadi Oskoee
6*1 Dental and Periodontal Research Center, Tabriz Medical Sciences University, Tabriz, Iran
2 Assistant Professor, Department of Operative Dentistry, Faculty of Dentistry, Tabriz Medical Sciences University, Tabriz, Iran
3 Professor, Department of Operative Dentistry, Faculty of Dentistry, Tabriz Medical Sciences University, Tabriz, Iran
4 Assistant Professor, Department of Oral Medicine, Faculty of Dentistry, Tabriz Medical Sciences University, Tabriz, Iran
5 Assistant Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tabriz Medical Sciences University, Tabriz, Iran
6 Post-graduate Student, Department of Oral and Maxillofacial Radiology, Faculty of Dentistry, Hamadan Medical Sciences University, Hamadan, Iran
Abstract
Background and aims. The aim was to evaluate the effect of casein phosphopeptide–amorphous calcium phosphate (CPP-ACP) on microtensile bond strength (µTBS) to carious affected dentin (CAD) using etch-and-rinse and self-etch adhesive systems. Materials and methods. The occlusal surface of 32 human molars with moderate occlusal caries was removed. Infected dentin was removed until reaching CAD and the teeth were randomly divided into two groups based on the Single Bond (SB) and Clearfil SE Bond (CSE) adhesive systems. Before composite resin bonding, each group was subdivided into three subgroups of ND, CAD and CPP-ACP-treated CAD (CAD-CPP) based on the dentin substrate. After dissecting samples to l-mm-thick cross-sections (each subgroup: n = 13), µTBS was measured at a strain rate of 0.5 mm/min. Data was analyzed using two-way ANOVA, independent samples t-test and post-hoc Tukey tests (α=0.05). Results. Bond strength of both adhesive systems to ND was significantly higher than that to CAD (P < 0.001) and CAD/CPP (P < 0.001). There were no significant differences between the µTBS of SB to CAD and CAD-CPP (P > 0.05). µTBS of CSE to CAD-CPP was higher than that to CAD; however, the difference was not significant (P > 0.05). Significant differences were found between SB and CSE systems only with CAD substrate (P < 0.001). Conclusion. Regardless of the adhesive system used, surface treatment of CAD with CPP-ACP did not have a significant effect on bond strength. However, bond strength to CAD was higher with SB rather than with CSE.