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J Dent Res Dent Clin Dent Prospects. 2015;9(2): 101-104.
doi: 10.15171/joddd.2015.020
PMID: 26236436
PMCID: PMC4517301
  Abstract View: 855
  PDF Download: 550

Original Research

Incidence Assessment of MTHFR C677T and A1298C Polymorphisms in Iranian Non-syndromic Cleft Lip and/or Palate Patients

Asghar Ebadifar 1*, Nazila Ameli 2, Hamid Reza Khorramkhorshid 3, Mehdi Salehi Zeinabadi4 4, Kourosh Kamali 5, Tayyebeh Khoshbakht 6

1 Associate Professor, Dentofacial Deformities Research Center, Research Institute of Dental Sciences, Shahid Behehsti University of Medical Sciences, Tehran, Iran
2 Assistant Professor of Orthodontics, Dental School, Semnan University of Medical Sciences, Semnan, Iran
3 Professor, Genetic Research Centre, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran
4 Assistant Professor of Pediatric Dentistry, Dental School, Semnan university of Medical Sciences, Semnan, Iran
5 Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
6 MSC, Genetic Research Centre, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran
*Corresponding Author: Email: ebadifar@hbi.ir

Abstract

Background and aims. The aim of the present study is to determine the incidence of MTHFR C677 T and A1298C muta-tions in Iranian patients with cleft lip and/or cleft palate.Materials and methods. We screened 61 Iranian patients with cleft lip and/or cleft palate for mutations in the two alleles of MTHFR gene associated with cleft lip and/or palate: A1298C and C677T, using Polymerase Chain Reaction following by RFLP.Results. The 677T and 1298C homozygote genotypes showed a frequency of 36.1% and 11.4%, respectively. Combined genotype frequencies in newborns having oral clefts showed that the highest genotype was 677TT/1298AA (22.9%) and 677TT/1298CC genotypes were not observed.Conclusion. The results showed that 65.6% of all patients had at least one T mutant allele in C677T and 58.9% C mutant allele for A1298C. According to the frequencies of homozygosity of mutant alleles, it could be said that MTHFRgenotype of 677TT shows a greater role in having oral clefts.
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Submitted: 21 Jun 2015
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