J Dent Res Dent Clin Dent Prospects. 2016;10(1): 43-47.
doi: 10.15171/joddd.2015.007
PMID: 25973152
PMCID: PMC4417491
  Abstract View: 619
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Original Research

Antimicrobial activity of tetraacetylethylenediamine-sodium perborate versus sodium hypochlorite against <italic>Enterococcus faecalis</italic>

Sahar Shakouie, Amin Salem Milani*, Mahsa Eskandarnejad, Saeed Rahimi, Mohammad Froughreyhani, Saeede Galedar, Ehsan Ranjbar


Background. This study evaluated the antimicrobial activity of Tetraacetylethylenediamine-sodium perborate (TAED-SP) in comparison to 2.5% and 5% sodium hypochlorite (NaOCl) against Enterococcus faecalis.Methods. A standard suspension of E. faecalis was inoculated on 60 plates containing Mueller-Hinton agar culture medium. Four sterile disks of Beckman filtration paper were placed on each plate. TAED-SP, 5% and 2.5% NaOCl were placed on three disks. Sterile physiologic saline was placed on the fourth disk as negative control. After 24-hour incubation, the diameter of the inhibition zone around the disks was measured using a transparent ruler. One-way Analysis of Variance (ANOVA) was used to compare the mean zone of microbial growth in the groups. P-values less than 0.05 were considered statistically significant.Results. There was a significant difference in the diameter of the inhibition zones between groups (P < 0.05). The Tukey post hoc test showed a higher diameter of the inhibitory zone with TAED-SP than that of 2.5% NaOCl. However, there were no significant differences between the inhibitory zones of TAED-SP and 5% NaOCl.Conclusion. TAED-SP and 5% NaOCl have similar antibacterial activity against E. faecalis; however, TAED-SP has a greater antibacterial effect compared to 2.5% NaOCl.
Keywords: Disk diffusion antimicrobial test, Enterococcus faecalis, sodium hypochlorite, tetraacetylethylenediamine
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Submitted: 05 Apr 2016
Accepted: 07 Apr 2016
ePublished: 07 Apr 2016
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