J Dent Res Dent Clin Dent Prospects. 2017;11(2): 73-77.
doi: 10.15171/joddd.2017.014
PMID: 28748046
PMCID: PMC5519996
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Basic Research

Original Article

Comparison of the effect of three autogenous bone harvesting methods on cell viability in rabbits

Janet Moradi Haghgoo 1, Seyed Reza Arabi 1, Seyyed Mohammad Hosseinipanah 2, Ghasem Solgi 3, Neda Rastegarfard 1*, Maryam Farhadian 4

1 Department of Periodontics, Faculty of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran
2 Department of Anatomical Science, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3 Department of Immunology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
4 Modeling of Noncommunicable Diseases Research Center, Department of Biostatistics, Faculty of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
*Corresponding Author; neda_rastegarfard@yahoo.com


Background. This study was designed to compare the viability of autogenous bone grafts, harvested using different methods, in order to determine the best harvesting technique with respect to more viable cells. Methods. In this animal experimental study, three harvesting methods, including manual instrument (chisel), rotary device and piezosurgery, were used for harvesting bone grafts from the lateral body of the mandible on the left and right sides of 10 rabbits. In each group, 20 bone samples were collected and their viability was assessed using MTS kit. Statistical analyses, including ANOVA and post hoc Tukey tests, were used for evaluating significant differences between the groups. Results. One-way ANOVA showed significant differences between all the groups (P=0.000). Data analysis using post hoc Tukey tests indicated that manual instrument and piezosurgery had no significant differences with regard to cell viability (P=0.749) and the cell viability in both groups was higher than that with the use of a rotary instrument (P=0.000). Conclusion. Autogenous bone grafts harvested with a manual instrument and piezosurgery had more viable cells in comparison to the bone chips harvested with a rotary device.
Keywords: Autograft, autologous transplants, dental Instruments, piezosurgery, tissue harvesting
© 2017 Moradi Haghgoo et al. This is an Open Access article published and distributed by Tabriz University of Medical Sciences under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Submitted: 21 Jul 2015
Accepted: 18 Jun 2017
ePublished: 21 Jun 2017
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