J Dent Res Dent Clin Dent Prospects. 2017;11(2):73-77.
doi: 10.15171/joddd.2017.014
PMID: 28748046
PMCID: PMC5519996
  Abstract View: 539
  PDF Download: 1148
  Full Text View: 63

Basic Research

Comparison of the effect of three autogenous bone harvesting methods on cell viability in rabbits

Janet Moradi Haghgoo 1, Seyed Reza Arabi 1, Seyyed Mohammad Hosseinipanah 2, Ghasem Solgi 3, Neda Rastegarfard 1 * , Maryam Farhadian 4

1 Department of Periodontics, Faculty of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran
2 Department of Anatomical Science, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3 Department of Immunology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
4 Modeling of Noncommunicable Diseases Research Center, Department of Biostatistics, Faculty of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
*Corresponding Author; Email: neda_rastegarfard@yahoo.com

Article

Background. This study was designed to compare the viability of autogenous bone grafts, harvested using different methods, in order to determine the best harvesting technique with respect to more viable cells. Methods. In this animal experimental study, three harvesting methods, including manual instrument (chisel), rotary device and piezosurgery, were used for harvesting bone grafts from the lateral body of the mandible on the left and right sides of 10 rabbits. In each group, 20 bone samples were collected and their viability was assessed using MTS kit. Statistical analyses, including ANOVA and post hoc Tukey tests, were used for evaluating significant differences between the groups. Results. One-way ANOVA showed significant differences between all the groups (P=0.000). Data analysis using post hoc Tukey tests indicated that manual instrument and piezosurgery had no significant differences with regard to cell viability (P=0.749) and the cell viability in both groups was higher than that with the use of a rotary instrument (P=0.000). Conclusion. Autogenous bone grafts harvested with a manual instrument and piezosurgery had more viable cells in comparison to the bone chips harvested with a rotary device.
© 2017 Moradi Haghgoo et al. This is an Open Access article published and distributed by Tabriz University of Medical Sciences under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
First name
 
Last name
 
Email address
 
Comments
 
Security code


Article Viewed: 539

Your browser does not support the canvas element.


PDF Downloaded: 1148

Your browser does not support the canvas element.


Full Text Viewed: 63

Your browser does not support the canvas element.

Submitted: 21 Jul 2015
Accepted: 18 Jun 2017
First published online: 21 Jun 2017
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - FireFox Plugin)