R. Banu Ermis 
1*, Esra Uzer CELIK 
2, Gul YILDIZ 
3, Basak YAZKAN 
41 Department of Restorative Dentistry, Faculty of Dentistry, Suleyman Demirel University, Isparta, Turkey
2 Department of Restorative Dentistry, Faculty of Dentistry, Izmir Katip Celebi University, Izmir, Turkey
3 Department of Restorative Dentistry, Faculty of Dentistry, Recep Tayyip Erdogan University, Rize, Turkey
4 Department of Restorative Dentistry, Faculty of Dentistry, Pamukkale University, Denizli, Turkey
        
 
        
	
        
        
Abstract
            Background. The use  of trayless at-home bleaching agents in darker teeth has raised some concerns  due to their unknown efficacy. The aim of this in vitro study was to evaluate the effect of tooth  discoloration severity on the efficacy and color stability of two different  trayless at-home bleaching systems.  Methods. Ninety enamel‒dentin samples were divided into  two groups: lighter and darker teeth. The teeth in each group were further  divided into three groups (n=15): (1) tray-based system with 10% carbamide  peroxide (Opalescence Oh) (control group); (2) a tray-applied whitening  membrane with 10% hydrogen peroxide (Treswhite Supreme); and (3) a whitening  pen with 22% carbamide peroxide (Hollywood Smiles). After bleaching, the  teeth were stained for 9 days using red wine. Color readings before bleaching  treatment were carried out using a dental spectrophotometer and were repeated  24 h after bleaching treatment and 24 h after staining process. ANOVA,  Bonferroni and Dunnett C tests were used at 5% significance level.  Results. No differences were observed between the bleaching  efficacy of Treswhite Supreme and Opalescence Oh in the lighter teeth, while  Opalescence Oh exhibited the best bleaching efficacy in the darker teeth  (P<0.05). Hollywood Smiles exhibited the worst bleaching efficacy but the  best color stability in both lighter and darker teeth (P<0.05). No  differences were observed between the color stability values of Treswhite  Supreme and Opalescence Oh.  Conclusion. This study suggested  that both the tooth discoloration severity and the type of trayless system used  affected the bleaching efficacy, whereas only the type of trayless system  affected the color stability.