J Dent Res Dent Clin Dent Prospects. 2020;14(2): 97-103.
doi: 10.34172/joddd.2020.022
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Basic Research

Original Article

Evaluation of the adhesion of human dental pulp stem cells to different endodontic biomaterials before and after setting

Marzie Aghazade 1 ORCID logo, Mohammad Samiei 2, Marjan Imani 3, Zahra Aghazadeh 1, Effat Alizadeh 4, Fereshte Rezaie 5* ORCID logo

1 Department of Oral Medicine, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran
2 Department of Endodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran
3 General Dental Practitioner, Khorram Abad, Iran
4 Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, University of Medical Sciences, Tabriz, Iran
5 General Dental Practitioner, Tabriz, Iran
*Corresponding authors: Fereshte Rezaie. Tel:+98- 04133355967 Email: rezaeefereshte20@gmail.com


Background. Stem cell-based treatment modalities have been potential strategies for tissue regeneration in many conditions. Several studies have evaluated the biologic properties of DPSCs and their efficacy in the treatment of a variety of diseases. The present study was undertaken to evaluate the adhesion behavior of DPSCs on different endodontic materials before and after setting.

Methods. The crowns of the selected teeth were removed, and the root canals were prepared and obturated with gutta-percha and AH26 sealer. A retrograde cavity was prepared at root ends. Different materials were placed in the cavities. Then the samples were attached to the wells with the use of a chemical glue. Dental pulp stem cells were allowed to proliferate to reach a count of 2 million and transferred to 12-well plates in association with a culture medium. Finally, the samples attached to the wells were exposed to the stem cells immersed in the culture medium before and after setting. Then adhesion of the stem cells was evaluated using SEM.

Results. The SEM results showed cellular adhesion in the samples containing CEM cement both before and after setting. The samples containing MTA Angelus and ProRoot MTA exhibited cellular adhesion before setting, with no cellular adhesion after setting. The samples containing AH26 and MTA Fillapex sealers exhibited cellular adhesion after setting, with no adhesion before setting. The samples containing simvastatin exhibited no cellular adhesion before setting; this material had dissolved in the culture medium after setting evaluation.

Conclusion. The results of the present study showed that of all the materials tested, CEM cement had the highest capacity for dental pulp stem cell adhesion.

Keywords: Adult stem cells, Biocompatible Materials, MTA, Regenerative Endodontics
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Submitted: 16 Apr 2019
Accepted: 14 Mar 2020
ePublished: 17 Jun 2020
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