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J Dent Res Dent Clin Dent Prospects. 2020;14(4): 214-217.
doi: 10.34172/joddd.2020.044
PMID: 33575009
PMCID: PMC7867686
Scopus ID: 85118420667
  Abstract View: 1009
  PDF Download: 662
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Basic Research

Intratubular Enterococcus faecalis viability assessment following root canal instrumentation with rotary and reciprocating systems via fluorescence microscopy

Jamileh Ghoddusi 1 ORCID logo, Ehsan Arian 2, Maryam Golmohammadi 3, Maryam Gharechahi 4* ORCID logo, Siavash Moushekhian 1

1 Dental Research Center, Faculty of Dentistry, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran
2 Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
3 Department of Endodontics, Khorasan Shomali University of Medical Sciences, Bojnord, Iran
4 Dental Materials Research Center, Faculty of Dentistry, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran
*Corresponding Author: *Corresponding author: Maryam Gharechahi, Email: , Email: gharechahim@mums.ac.ir

Abstract

Background. The present in vitro study aimed to compare the effectiveness of the WaveOne and ProTaper Gold systems in removing the Enterococcus faecalis biofilm.

Methods. Thirty-eight mandibular premolars were selected. The root canals were assigned to standard control (canals serially enlarged with ProTaper Gold S1-S2-F1-F2, n=15) and experimental (canals enlarged with Primary WaveOne file, n=15) groups. Following the instrumentation procedure, the root canals underwent a sampling procedure, and the colonyforming unit (CFU) counts were determined. The samples were also evaluated under a fluorescent microscope to evaluate viable bacteria. The data were analyzed using independent samples t test and paired samples t test.

Results. The results showed that, compared with the ProTaper group, the WaveOne group exhibited the least viable bacteria (P=0.004).

Conclusion. It was concluded that comparison with the ProTaper Gold rotary system, the WaveOne reciprocating file is more successful in reducing intratubular viable bacteria counts.

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Submitted: 07 Jul 2020
Accepted: 17 Sep 2020
ePublished: 02 Dec 2020
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