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J Dent Res Dent Clin Dent Prospects. 2021;15(3): 152-156.
doi: 10.34172/joddd.2021.026
  Abstract View: 132
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Basic Research

Original Article

Cytotoxicity assessment of Bio-C Repair Íon+: A new calcium silicate-based cement

Celso Afonso Klein-Junior 1,2 ORCID logo, Roberto Zimmer 1* ORCID logo, Tãnyre Dobler 2 ORCID logo, Vanessa Oliveira 2 ORCID logo, Daniel Rodrigo Marinowic 3 ORCID logo, Ahmet Özkömür 1 ORCID logo, Eduardo Galia Reston 1 ORCID logo

1 Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil, Canoas, RS, Brazil
2 Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil, Cachoeira do Sul, RS, Brazil
3 Brain Institute, Neuroscience laboratory, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil
*Corresponding author: Roberto Zimmer, Email: beto.zimmer@hotmail.com

Abstract

Background. Direct pulp capping is a method designed to preserve the exposed dental pulp. Due to good biological, physical, and mechanical properties, new versions of calcium silicate-based materials have been developed as pulp capping materials. The present study aimed to evaluate the cytotoxic effects of four calcium silicate-based pulp capping materials, of which the Bio-C Repair Íon+ is still in an experimental phase.

Methods. Biodentine, MTA Repair HP, Bio-C Repair, and Bio-C Repair Íon+ cements were dispensed in a metallic matrix to produce 125-mm3 specimens, which were immersed in Dulbecco’s Modified Eagle Medium (DMEM) to obtain extracts. NIH 3T3 cells were cultured and exposed to the extracts for 24 hours and seven days. Cell viability was assessed by the methyl tetrazolium test (MTT). The mean values for the experimental and control groups (without treatment) were compared by analysis of variance (ANOVA) and post hoc Tukey tests, considering a significance level of 5%.

Results. All the tested materials demonstrated a reduction in cell viability (P<0.05). According to ISO 10993-5: 2009 (E), Bio-C Repair Íon+ exhibited mild and moderate cytotoxicity in the 24- hour and 7-day analyses, respectively. Bio-C Repair and Biodentine showed mild cytotoxicity, and MTA Repair HP exhibited moderate cytotoxicity at both intervals.

Conclusion. The highest cell viability was demonstrated by Biodentine, MTA, and Repair HP, in descending order. Bio-C Repair and Bio-C Repair Íon+ showed moderate cytotoxicity, similar to MTA Repair HP in the 7-day analysis.


Keywords: Calcium silicate, Cell survival, Fibroblasts, Mineral trioxide aggregate

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Submitted: 26 Dec 2020
Accepted: 18 Apr 2021
ePublished: 25 Aug 2021
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