Logo-joddd
J Dent Res Dent Clin Dent Prospects. 2023;17(4): 242-249.
doi: 10.34172/joddd.2023.39163
  Abstract View: 260
  PDF Download: 197

Basic Research

Original Article

In vivo cytotoxicity analysis of bioceramic root canal sealers on zebrafish embryo

Siti Aisyah Nadirah Ja'apar 1* ORCID logo, Solachuddin Jauhari Arief Ichwan 2,3, Musliana Mustaffa 4

1 Department of Biotechnology, Kulliyyah of Sciences, International Islamic University Malaysia (IIUM), Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia
2 Department of Fundamental Dental and Medical Sciences, Kulliyyah of Dentistry (KOD), IIUM, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia
3 Dentistry Programme, PAPRSB Institute of Health Sciences, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, BE1410, Brunei
4 Department of Restorative Dentistry, KOD, IIUM, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia
*Corresponding Author: Siti Aisyah Nadirah Ja’apar, Email: ctaisyahnadirah97@gmail.com

Abstract

Background. This study evaluated the cytotoxicity of four bioceramic root canal sealers (RCSs) in vivo. The embryonic zebrafish characteristics, such as mortality, survival, hatching, and general morphology, served as the parameters for assessing cytotoxicity.

Methods. The RCSs, namely GuttaFlow Bioseal, MTA Fillapex, CeraSeal Bioceramic, and iRoot SP, were mixed according to the manufacturer’s guidelines. The extract solution was prepared by immersing the set RCS into 1X dilution of E3 solution. Then, the extract solution was delivered into a Petri dish where zebrafish embryos were allowed to develop. Cytotoxicity was evaluated 24, 48, 72, and 96 hours after fertilization.

Results. The Kruskal-Wallis test showed that except for GuttaFlow Bioseal, the mortality, survival, and hatching of zebrafish embryos for the remaining three bioceramic RCSs were significantly different from the negative controls (P<0.05). Significant differences were also evident in the mortality, survival, and hatching of zebrafish embryos between GuttaFlow Bioseal and three other RCSs (P<0.05).

Conclusion. GuttaFlow Bioseal was less cytotoxic than other bioceramics RCSs; MTA Fillapex, CeraSeal Bioceramic root canal sealer, and iRoot SP root canal sealer exhibited comparable cytotoxicity.

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 261

Your browser does not support the canvas element.


PDF Download: 197

Your browser does not support the canvas element.

Submitted: 13 Mar 2023
Accepted: 13 Nov 2023
ePublished: 30 Dec 2023
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)