J Dent Res Dent Clin Dent Prospects. 2024;18(1): 23-28.
doi: 10.34172/joddd.40705
  Abstract View: 196
  PDF Download: 183

Clinical Dentistry

Original Article

Antibacterial effects of children’s and adults’ toothpastes containing different amounts of fluoride: An in vitro study

Arthit Klaophimai 1 ORCID logo, Orada Tosrisawatkasem 1, Sivaporn Horsophonphong 2* ORCID logo

1 Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
2 Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
*Corresponding Author: Sivaporn Horsophonphong, Email: sivaporn.hor@mahidol.edu


Background. In recent years, fluoride concentrations in toothpaste for children and adults have increased. However, the effects of different concentrations on bacterial activity have rarely been compared. We aimed to investigate and compare the antibacterial activity of children’s and adults’ toothpaste containing 500, 1000‒1100, and 1450‒1500 ppm fluoride.

Methods. Three strains of bacteria (Streptococcus mutans, Streptococcus salivarius, and Lactobacillus casei) were cultured in brain heart infusion agar. Thirty commercially available toothpaste products for children and adults containing 500, 1000‒1100, and 1450‒1500 ppm fluoride were selected and tested. Toothpaste’s ability to inhibit bacterial growth was evaluated by agar diffusion assay, in which plates were incubated for 24 hours, and then the diameter of the microbial inhibition zone was measured. Comparisons between children’s and adults’ fluoride toothpastes were made using the Mann-Whitney U test. The association between bacterial growth inhibition and sodium lauryl sulfate (SLS) was analyzed by the chi-square test. A P value of <0.05 was considered statistically significant.

Results. No difference in the inhibition zone was observed for different fluoride concentrations. However, there were significant differences between toothpastes for children and adults, with higher inhibition zones for adults’ toothpastes. Most toothpastes for adults contained SLS, which was associated with antibacterial activity.

Conclusion. Fluoride concentrations ranging from 500 to 1500 ppm did not affect bacterial growth. The antibacterial activity of toothpastes for adults was significantly higher than that of toothpastes for children, which was mainly attributed to the SLS usually added to adult formulations.

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Submitted: 23 Sep 2023
Accepted: 03 Jan 2024
ePublished: 29 Mar 2024
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