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J Dent Res Dent Clin Dent Prospects. 2025;19(3): 160-171.
doi: 10.34172/joddd.025.43844
  Abstract View: 60
  PDF Download: 40

Basic Research

Original Article

Impact of adhesive layer addition on the optical properties of remineralized white spot lesions

Francesca Zotti 1 ORCID logo, Giorgia Lanzaretti 2* ORCID logo, Francesca Pilati 3, Nicoletta Zerman 1 ORCID logo

1 Department of Surgical Sciences, Pediatrics and Gynecology, University of Verona, P.le L.A. Scuro 10, 37134 Verona, Italy
2 Private Practice, Vicenza, Italy
3 Private Practice, Trento, Italy
*Corresponding Author: Giorgia Lanzaretti, Email: lanzarettigiorgia@gmail.com

Abstract

Background. The present in vitro study evaluated the camouflage effect of two treatments for white spot lesions (WSLs): remineralization with Mi Paste Plus© (GC Europe N.V.) and remineralization combined with a Scotchbond Multi-Purpose© (3M ESPE 2015) adhesive layer. Additionally, the study assessed the color stability of the treatments when exposed to a staining agent, such as coffee.

Methods. Sixty extracted anterior and posterior teeth were preserved in saline solution and divided into two groups. Artificial WSLs were created using 37% orthophosphoric acid for 1 minute. Both groups underwent daily 6-hour remineralization treatments with Mi Paste Plus© for 14 days. In the second group, a thin adhesive layer was applied after remineralization. Color analysis was performed using an MHT SpectroShade Micro spectrophotometer at baseline (T1 ), after remineralization (T2 ), after adhesive application for group 2 (TR), and after immersion in coffee for 24 hours (T3 ). Color differences (ΔE) between healthy tissue and WSLs were assessed, with ΔE≤3.3 considered clinically acceptable. Data were analyzed using STATA 17 (P≤0.05).

Results. ΔE analysis showed a statistically significant improvement in the camouflage effect in the adhesive-treated group (TR) compared to remineralization alone (T2 ). After coffee exposure (T3 ), ΔE values increased significantly in both groups, with higher values observed in the adhesive-treated group. Intragroup analyses indicated significant differences between T2 and T3 , as well as TR and T3 .

Conclusion. The application of an adhesive system can be considered a valid option to improve the aesthetic outcome of a remineralization treatment; however, this approach carries the risk of resin staining over time.


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Submitted: 06 Jul 2025
Accepted: 24 Aug 2025
ePublished: 30 Sep 2025
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