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J Dent Res Dent Clin Dent Prospects. 2025;19(4): 215-221.
doi: 10.34172/joddd.025.43876
  Abstract View: 12
  PDF Download: 10

Clinical Dentistry

Original Article

Effect of single and repeated potassium iodide applications versus glutathione on silver diamine fluoride-induced tooth discoloration in primary teeth: An in vitro comparative study

Maryam Hajiahmadi 1 ORCID logo, Samira SadighBatha 2* ORCID logo, Mohammad Javad Tarrahi 3 ORCID logo

1 Department of Pediatric Dentistry, Dental Research Center, Faculty of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
2 Dental Students’ Research Committee, Department of Pediatric Dentistry, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Epidemiology and Biostatistics, School of Health, Isfahan University of Medical Science, Isfahan, Iran
*Corresponding Author: Samira SadighBatha, Email: samirasadighbatha@gmail.com

Abstract

Background. This study aimed to evaluate the effects of a single application of potassium iodide (KI), repeated daily applications of KI, and glutathione (GSH) on silver diamine fluoride (SDF)-induced tooth discoloration in primary canines.

Methods. This in vitro study was performed on 36 extracted primary canines with dentinal caries, which were randomly assigned to four treatment groups: A (38% SDF alone), B (38% SDF+immediate single application of KI), C (38% SDF+repeated daily KI applications), and D (38% SDF+20% GSH). Color changes were measured at baseline and after 1, 14, and 30 days using a spectrophotometer and the CIEL*a*b* system. Statistical analysis included one-way ANOVA with post hoc Tukey tests for inter-group comparisons, and repeated-measures ANOVA with Bonferroni’s correction for intra-group comparisons.

Results. Significant differences in tooth discoloration (ΔE) were observed between the study groups at all time intervals (P<0.001). Group A showed the highest discoloration, while group D exhibited the lowest. Among the KI-treated groups, group B showed progressive discoloration over time, while group C demonstrated minimal changes, with no statistically significant differences between time intervals (P=0.435). Although groups C and D both showed reduced discoloration compared to group B, the difference between them was not statistically significant on day 30 (P=0.073).

Conclusion. Repeated daily applications of KI or GSH effectively reduced SDF-induced tooth discoloration in primary canines. GSH showed the most favorable esthetic outcomes, suggesting its potential as a promising adjunct to SDF.




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Submitted: 15 Jul 2025
Revision: 03 Oct 2025
Accepted: 16 Oct 2025
ePublished: 31 Dec 2025
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