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J Dent Res Dent Clin Dent Prospects. 2026;20(1): 25-31.
doi: 10.34172/joddd.44162
  Abstract View: 47
  PDF Download: 34

Basic Research

Original Article

Effects of Calcium Silicate-Based Sealer Residues on Adhesive Bonding to Coronal Dentin: An in Vitro Study

Mariana Bena Gelio 1 ORCID logo, Thais Piragine Leandrin 1 ORCID logo, Ana Lídia Pinheiro Silva Sato 1 ORCID logo, Milton Carlos Kuga 1 ORCID logo, Joissi Ferrari Zaniboni 2* ORCID logo

1 Department of Restorative Dentistry, Araraquara School of Dentistry, São Paulo State University - UNESP, Araraquara, SP, Brazil
2 Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil
*Corresponding Author: Joissi Ferrari Zaniboni, Email: joissi.zaniboni@usp.br

Abstract

Introduction: This study aimed to evaluate the influence of residues from three endodontic sealers on the adhesive interface of two universal adhesive systems: Scotchbond Universal (SU) and OptiBond All-in-One (OU).

Methods: Forty bovine dentin surfaces were cleaned with 95% ethanol and divided into 4 groups: control, without endodontic sealer; AH, dentin impregnated with AH PLUS sealer; ES, EndoSequence BC Sealer; and MTA, MTA Fillapex. After cleaning, the persistence of residues and the number of open dentinal tubules were analyzed by scanning electron microscopy. Sixty bovine incisor crowns were divided into six groups (n=10): AH-SU, ES-SU, MTA-SU, AH-OU, ES-OU, and MTA-OU to evaluate the microshear bond strength in a universal testing machine and failure mode, in stereomicroscopy. Data were analyzed using the Kruskal-Wallis test, ANOVA, and post hoc Tukey tests (α=0.05).

Results: AH Plus showed higher persistence of residues and fewer open tubules compared to other groups (P<0.05), whereas EndoSequence BC Sealer and MTA Fillapex did not differ from each other (P>0.05). Bond strength was lower in AH Plus groups, regardless of the adhesive system used (P<0.05). EndoSequence BC Sealer and MTA Fillapex exhibited similar and higher bond strength than AH Plus (P>0.05). Adhesive failure was the most frequent mode in all groups, except for MTA-SU, which showed a higher incidence of mixed failures.

Conclusion: Residues of endodontic sealers affect dentinal penetrability and the adhesion of universal adhesives. In the present study, EndoSequence BC Sealer and MTA Fillapex had less impact on the adhesive interface than AH Plus, suggesting a lower impact on adhesion after cleaning with 95% ethanol.


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Submitted: 19 Sep 2025
Revision: 03 Dec 2025
Accepted: 24 Dec 2025
ePublished: 30 Mar 2026
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