J Dent Res Dent Clin Dent Prospects. 2017;11(1): 14-17.
doi: 10.15171/joddd.2017.003
PMID: 28413590
PMCID: PMC5390120
  Abstract View: 2698
  PDF Download: 2640

Basic Research

Original Article

Effect of nano-hydroxyapatite toothpaste on microhardness of artificial carious lesions created on extracted teeth

Asghar Ebadifar 1*, Mohammad Nomani 2, Sayed Ali Fatemi 3

1 Dentofacial Deformities Research Center, Research Institute of Dental Sciences & Department of Orthodontics, Dental School, Shahid Behehsti Univer-sity of Medical Sciences, Tehran, Iran
2 Dentist, Private Practice, Tehran, Iran
3 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
*Corresponding Author: *Corresponding Author; , Email: ebadifar@hbi.ir


Background. Many types of toothpastes contain substances that can remineralize initial enamel caries. This study aimed to assess the effect of nano-hydroxyapatite (NHA) on microhardness of artificially created carious lesions.

Methods. In this in vitro study, NHA was prepared using sol-gel technique and added to the toothpaste with 7% concentra-tion. A total of 80 extracted sound teeth were collected. The crowns were polished using 500-grit abrasive paper. The spe-cimens were randomly coded from 1 to 80. Number 1 to 40 were assigned to group A and numbers 41 to 80 to group B. The microhardness was measured using HVS-1000 Vickers microhardness tester. The specimens were demineralized using 37% phosphoric acid for 3 minutes in order to create artificial carious lesions and then were rinsed with water, air-sprayed for 3 minutes and dried. Microhardness was measured again. Next, the specimens were brushed for 15 days, twice daily, for 15 seconds. After 15 days, microhardness was measured again. Toothpaste A contained NHA and fluoride and toothpaste B contained fluoride alone. Data were analyzed using SPSS 16, with one-sample Kolmogorov-Smirnov test and ANOVA at a significance level of P<0.05.

Results. The microhardness of specimens significantly decreased following acid exposure (P<0.01) but increased again in both groups after exposure to toothpastes. The increase in microhardness was significantly greater in group A (P<0.01).

Conclusion. The toothpaste containing NHA was more effective than the toothpaste without NHA for the purpose of re-mineralization.

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Submitted: 14 Aug 2016
Accepted: 01 Mar 2017
ePublished: 15 Mar 2017
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