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J Dent Res Dent Clin Dent Prospects. 2023;17(3): 188-195.
doi: 10.34172/joddd.2023.37106
PMID: 38023802
PMCID: PMC10676540
  Abstract View: 488
  PDF Download: 364

Basic Research

Original Article

The association between oral inflammatory load and semen and sperm functional analysis: A cross-sectional study

Reza Pourabbas 1,2 ORCID logo, Samin Farajzadeh 1* ORCID logo, Amirreza Babaloo 1, Azar Pazhohan 3, Mehrnoosh Sadighi 1, Sakineh Hajebrahimi 4, Sajjad Pourabbas 5, Howard C Tenenbaum 6,7

1 Department of Periodontology, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran
2 Dental and Periodontal Research Center, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran
3 Omid Infertility Treatment Center, Valiasr Hospital, Tabriz, Iran
4 Research Center for Evidence-based Medicine, Urology Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
5 School of Kinesiology and Health Sciences, York University, Toronto, Canada
6 Faculty of Dentistry, University of Toronto, Toronto, Canada
7 Faculty of Medicine, University of Toronto, Toronto, Canada
*Corresponding Author: Samin Farajzadeh, Email: saminfarajzadeh78@gmail.com

Abstract

Background. Studies have suggested a correlation between periodontitis and reduced male fertility. Inflammation has been described as the link between these ailments. Oral inflammatory load (OIL) can be measured through oral polymorphonuclear neutrophil (oPMN) count, which is associated with periodontal diseases. This cross-sectional study assessed the possible correlation between OIL and the functional parameters of sperm cells.

Methods. In 229 volunteers, oral rinse and semen samples were assessed for oPMN, semen polymorphonuclears (sPMNs), sperm concentration, total sperm count, motility, morphology, and sperm DNA fragmentation index (SDFi). A multiple linear regression model was conducted to evaluate the relationships between oPMN and semen parameters.

Results. The effect of elevated oPMN counts on total motility rate, progressive rate, and percentage of sperm cells with normal morphology was significant (P<0.001), with an inverse relationship, i.e., with every unit increase in oPMN count, the mentioned parameters would decline by 0.573, 0.367, and 0.407 units, respectively. oPMN counts also correlated positively with sPMN counts and SDFi (P<0.001), i.e., with every unit increase in the oPMN measures, sPMN counts would increase by 0.126 million/mL, with an 0.733% increase in SDFi. However, there was no significant association between oPMN counts and sperm concentration.

Conclusion. OIL, as represented by oPMN counts, might affect male fertility as there is a positive correlation between the levels of these inflammatory cells and decreased sperm motility, abnormal morphological changes, increased sPMN counts, and increased SDFi.

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Submitted: 26 Feb 2023
Revision: 09 Jul 2023
Accepted: 15 Aug 2023
ePublished: 11 Nov 2023
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